                         SEQUENCE LISTING

<110>  Transalgae Ltd
 
<120>  Improving the content of the essential amino acids lysine and 
       methionine in algae and cyanobacteria for improved animal feed

<130>  Transalgae Fishmeal

<150>  12,658,815
<151>  2010-02-16

<150>  12/584,571
<151>  2009-09-08

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  909
<212>  DNA
<213>  Corynebacterium


<220>
<221>  CDS
<222>  (1)..(909)
<223>  coding region of feedback insensitive bacterial DHDPS

<400>  1
atg agc aca ggt tta aca gct aag acc gga gta gag cac ttc ggc acc         48
Met Ser Thr Gly Leu Thr Ala Lys Thr Gly Val Glu His Phe Gly Thr           
1               5                   10                  15                

gtt gga gta gca atg gtt act cca ttc acg gaa tcc gga gac atc gat         96
Val Gly Val Ala Met Val Thr Pro Phe Thr Glu Ser Gly Asp Ile Asp           
            20                  25                  30                    

atc gct gct ggc cgc gaa gtc gcg gct tat ttg gtt gat aag ggc ttg        144
Ile Ala Ala Gly Arg Glu Val Ala Ala Tyr Leu Val Asp Lys Gly Leu           
        35                  40                  45                        

gat tct ttg gtt ctc gcg ggc acc act ggt gaa tcc cca acg aca acc        192
Asp Ser Leu Val Leu Ala Gly Thr Thr Gly Glu Ser Pro Thr Thr Thr           
    50                  55                  60                            

gcc gct gaa aaa cta gaa ctg ctc aag gcc gtt cgt gag gaa gtt ggg        240
Ala Ala Glu Lys Leu Glu Leu Leu Lys Ala Val Arg Glu Glu Val Gly           
65                  70                  75                  80            

gat cgg gcg aag ctc atc gcc ggt gtc gga acc aac aac acg cgg aca        288
Asp Arg Ala Lys Leu Ile Ala Gly Val Gly Thr Asn Asn Thr Arg Thr           
                85                  90                  95                

tct gtg gaa ctt gcg gaa gct gct gct tct gct ggc gca gac ggc ctt        336
Ser Val Glu Leu Ala Glu Ala Ala Ala Ser Ala Gly Ala Asp Gly Leu           
            100                 105                 110                   

tta gtt gta act cct tat tac tcc aag ccg agc caa gag gga ttg ctg        384
Leu Val Val Thr Pro Tyr Tyr Ser Lys Pro Ser Gln Glu Gly Leu Leu           
        115                 120                 125                       

gcg cac ttc ggt gca att gct gca gca aca gag gtt cca att tgt ctc        432
Ala His Phe Gly Ala Ile Ala Ala Ala Thr Glu Val Pro Ile Cys Leu           
    130                 135                 140                           

tat gac att cct ggt cgg tca ggt att cca att gag tct gat acc atg        480
Tyr Asp Ile Pro Gly Arg Ser Gly Ile Pro Ile Glu Ser Asp Thr Met           
145                 150                 155                 160           

aga cgc ctg agt gaa tta cct acg att ttg gcg gtc aag gac gcc aag        528
Arg Arg Leu Ser Glu Leu Pro Thr Ile Leu Ala Val Lys Asp Ala Lys           
                165                 170                 175               

ggt gac ctc gtt gca gcc acg tca ttg atc aaa gaa acg gga ctt gcc        576
Gly Asp Leu Val Ala Ala Thr Ser Leu Ile Lys Glu Thr Gly Leu Ala           
            180                 185                 190                   

tgg tat tca ggc gat gac cca cta aac ctt gtt tgg ctt gct ttg ggc        624
Trp Tyr Ser Gly Asp Asp Pro Leu Asn Leu Val Trp Leu Ala Leu Gly           
        195                 200                 205                       

gga tca ggt ttc att tcc gta att gga cat gca gcc ccc aca gca tta        672
Gly Ser Gly Phe Ile Ser Val Ile Gly His Ala Ala Pro Thr Ala Leu           
    210                 215                 220                           

cgt gag ttg tac aca agc ttc gag gaa ggc gac ctc gtc cgt gcg cgg        720
Arg Glu Leu Tyr Thr Ser Phe Glu Glu Gly Asp Leu Val Arg Ala Arg           
225                 230                 235                 240           

gaa atc aac gcc aaa cta tca ccg ctg gta gct gcc caa ggt cgc ttg        768
Glu Ile Asn Ala Lys Leu Ser Pro Leu Val Ala Ala Gln Gly Arg Leu           
                245                 250                 255               

ggt gga gtc agc ttg gca aaa gct gct ctg cgt ctg cag ggc atc aac        816
Gly Gly Val Ser Leu Ala Lys Ala Ala Leu Arg Leu Gln Gly Ile Asn           
            260                 265                 270                   

gta gga gat cct cga ctt cca att atg gct cca aat gag cag gaa ctt        864
Val Gly Asp Pro Arg Leu Pro Ile Met Ala Pro Asn Glu Gln Glu Leu           
        275                 280                 285                       

gag gct ctc cga gaa gac atg aaa aaa gct gga gtt cta ctc gag            909
Glu Ala Leu Arg Glu Asp Met Lys Lys Ala Gly Val Leu Leu Glu               
    290                 295                 300                           


<210>  2
<211>  303
<212>  PRT
<213>  Corynebacterium

<400>  2

Met Ser Thr Gly Leu Thr Ala Lys Thr Gly Val Glu His Phe Gly Thr 
1               5                   10                  15      


Val Gly Val Ala Met Val Thr Pro Phe Thr Glu Ser Gly Asp Ile Asp 
            20                  25                  30          


Ile Ala Ala Gly Arg Glu Val Ala Ala Tyr Leu Val Asp Lys Gly Leu 
        35                  40                  45              


Asp Ser Leu Val Leu Ala Gly Thr Thr Gly Glu Ser Pro Thr Thr Thr 
    50                  55                  60                  


Ala Ala Glu Lys Leu Glu Leu Leu Lys Ala Val Arg Glu Glu Val Gly 
65                  70                  75                  80  


Asp Arg Ala Lys Leu Ile Ala Gly Val Gly Thr Asn Asn Thr Arg Thr 
                85                  90                  95      


Ser Val Glu Leu Ala Glu Ala Ala Ala Ser Ala Gly Ala Asp Gly Leu 
            100                 105                 110         


Leu Val Val Thr Pro Tyr Tyr Ser Lys Pro Ser Gln Glu Gly Leu Leu 
        115                 120                 125             


Ala His Phe Gly Ala Ile Ala Ala Ala Thr Glu Val Pro Ile Cys Leu 
    130                 135                 140                 


Tyr Asp Ile Pro Gly Arg Ser Gly Ile Pro Ile Glu Ser Asp Thr Met 
145                 150                 155                 160 


Arg Arg Leu Ser Glu Leu Pro Thr Ile Leu Ala Val Lys Asp Ala Lys 
                165                 170                 175     


Gly Asp Leu Val Ala Ala Thr Ser Leu Ile Lys Glu Thr Gly Leu Ala 
            180                 185                 190         


Trp Tyr Ser Gly Asp Asp Pro Leu Asn Leu Val Trp Leu Ala Leu Gly 
        195                 200                 205             


Gly Ser Gly Phe Ile Ser Val Ile Gly His Ala Ala Pro Thr Ala Leu 
    210                 215                 220                 


Arg Glu Leu Tyr Thr Ser Phe Glu Glu Gly Asp Leu Val Arg Ala Arg 
225                 230                 235                 240 


Glu Ile Asn Ala Lys Leu Ser Pro Leu Val Ala Ala Gln Gly Arg Leu 
                245                 250                 255     


Gly Gly Val Ser Leu Ala Lys Ala Ala Leu Arg Leu Gln Gly Ile Asn 
            260                 265                 270         


Val Gly Asp Pro Arg Leu Pro Ile Met Ala Pro Asn Glu Gln Glu Leu 
        275                 280                 285             


Glu Ala Leu Arg Glu Asp Met Lys Lys Ala Gly Val Leu Leu Glu 
    290                 295                 300             


<210>  3
<211>  448
<212>  PRT
<213>  Chlamydomonas reinhardtii


<220>
<221>  PEPTIDE
<222>  (1)..(448)
<223>  lysine-ketoglutarate reductase/saccharopine dehydrogenase

<400>  3

Met Arg Arg Val Ala Asn Thr Ser Arg Ala Thr Gly Ala Arg Cys Gln 
1               5                   10                  15      


Gly Ala Lys Leu Val Ala Arg Pro Cys Ala Arg Arg Ala Ala Val His 
            20                  25                  30          


Val Ile Cys Ala Thr Gly Pro Val Pro Asp Lys Ser Val Val Val Ile 
        35                  40                  45              


Gly Gly Thr Gly Arg Val Gly Ser Ser Thr Ala Ala Thr Leu Leu Lys 
    50                  55                  60                  


Glu Phe Pro Asn Leu Lys Val Thr Val Ala Ser Arg Ser Asp Asp Ser 
65                  70                  75                  80  


Phe Lys Ala Ala Val Glu Arg Arg Pro Glu Leu Ser Lys Ala Gly Phe 
                85                  90                  95      


Gln Arg Val Asp Ile Thr Asn Ala Asp Ser Val Gln Ala Leu Leu Lys 
            100                 105                 110         


Ser Thr Gly Ala Asp Leu Val Ile His Thr Ala Gly Pro Phe Gln Arg 
        115                 120                 125             


Ser Lys Asn Tyr Ala Val Leu Glu Ala Ala Ile Ala Ser Gly Thr Gly 
    130                 135                 140                 


Tyr Ile Asp Val Cys Asp Asp Thr Pro Phe Ala Glu Gly Ala Lys Ala 
145                 150                 155                 160 


Ala Tyr Met Glu Lys Ala Lys Ala Ala Gly Val Pro Ala Ile Val Ser 
                165                 170                 175     


Gly Gly Ile Tyr Pro Gly Thr Ser Asn Val Met Ala Ala His Ile Ile 
            180                 185                 190         


Ser Ile Ala Arg Ala Glu Tyr Asp Asp Asn Trp Asn Tyr Arg Thr Pro 
        195                 200                 205             


Ala Pro Gly Glu Ser Val Glu Pro Lys Trp Leu Arg Tyr Ser Tyr Tyr 
    210                 215                 220                 


Thr Ala Gly Ser Gly Gly Ala Gly Pro Thr Ile Leu Glu Thr Ser Phe 
225                 230                 235                 240 


Leu Leu Ala Gly Glu Asp Val Ile Val Tyr Lys Asp Asn Lys Glu Val 
                245                 250                 255     


Val Leu Pro Pro Ile Ser Asn Arg Arg Glu Val Asp Phe Gly Pro Gly 
            260                 265                 270         


Val Gly Arg Lys Gly Val Tyr Leu Tyr Asn Leu Pro Glu Val Val Ser 
        275                 280                 285             


Gly His Lys Tyr Met Arg Val Pro Asp Val Ser Ala Arg Phe Gly Thr 
    290                 295                 300                 


Asp Pro Phe Ile Trp Asn Trp Ala Met Trp Leu Thr Ala Arg Leu Val 
305                 310                 315                 320 


Pro Arg Ser Leu Leu Asn Asp Arg Asn Phe Val Lys Gly Phe Ala Lys 
                325                 330                 335     


Leu Ser Asp Pro Phe Val Arg Asn Val Asp Lys Ile Ile Gly Glu Ala 
            340                 345                 350         


Val Ala Met Arg Val Glu Val Asp Met Val Gly Gly Lys Asn Ser Ser 
        355                 360                 365             


Gly Ile Phe Val His Lys Tyr Leu Ser Gln Ser Met Gly Tyr Ser Thr 
    370                 375                 380                 


Ala Ala Phe Ala Gln Ser Val Leu Gln Gly Lys Thr Gln Pro Gly Val 
385                 390                 395                 400 


Trp Tyr Pro Glu Glu Lys Glu Ala Leu Gln Asp Arg Arg Gln Phe Leu 
                405                 410                 415     


Gln Phe Ala Ala Thr Gly Cys Ser Arg Phe Glu Leu Asn Arg Ser Ala 
            420                 425                 430         


Trp Ala Leu Glu Ser Glu Ile Lys Gln Ile Gly Gly Met Ile Tyr Trp 
        435                 440                 445             


<210>  4
<211>  67
<212>  PRT
<213>  Chlamydomonas reinhardtii


<220>
<221>  PEPTIDE
<222>  (1)..(67)
<223>  BHL8

<400>  4

Met Ala Lys Met Lys Cys Thr Trp Pro Glu Leu Val Gly Lys Thr Val 
1               5                   10                  15      


Glu Lys Ala Lys Lys Met Ile Met Lys Asp Lys Pro Glu Ala Lys Ile 
            20                  25                  30          


Met Val Leu Pro Val Gly Thr Lys Val Thr Gly Glu Trp Lys Met Asp 
        35                  40                  45              


Arg Val Arg Leu Trp Val Asp Lys Lys Asp Lys Ile Ala Lys Thr Pro 
    50                  55                  60                  


Lys Cys Gly 
65          


<210>  5
<211>  147
<212>  PRT
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  PEPTIDE
<222>  (1)..(147)
<223>  synthetic alfa-helical coiled coil high lysine protein

<400>  5

Ala Thr Gly Gly Ala Gly Gly Ala Gly Ala Ala Gly Cys Thr Gly Ala 
1               5                   10                  15      


Ala Gly Gly Cys Cys Ala Thr Gly Gly Ala Gly Gly Ala Gly Ala Ala 
            20                  25                  30          


Gly Cys Thr Gly Ala Ala Gly Gly Cys Cys Ala Thr Gly Gly Ala Gly 
        35                  40                  45              


Gly Ala Gly Ala Ala Gly Cys Thr Gly Ala Ala Gly Gly Cys Cys Ala 
    50                  55                  60                  


Thr Gly Gly Ala Gly Gly Ala Gly Ala Ala Gly Cys Thr Gly Ala Ala 
65                  70                  75                  80  


Gly Gly Cys Cys Ala Thr Gly Gly Ala Gly Gly Ala Gly Ala Ala Gly 
                85                  90                  95      


Cys Thr Gly Ala Ala Gly Gly Cys Cys Ala Thr Gly Gly Ala Gly Gly 
            100                 105                 110         


Ala Gly Ala Ala Gly Cys Thr Gly Ala Ala Gly Gly Cys Cys Ala Thr 
        115                 120                 125             


Gly Gly Ala Gly Gly Ala Gly Ala Ala Gly Ala Thr Gly Ala Ala Gly 
    130                 135                 140                 


Gly Cys Cys 
145         


<210>  6
<211>  304
<212>  PRT
<213>  Amaranthus hypochondriacus


<220>
<221>  PEPTIDE
<222>  (1)..(304)
<223>  seed protein AmA1

<400>  6

Met Ala Gly Leu Pro Val Ile Met Cys Leu Lys Ser Asn Asn Asn Gln 
1               5                   10                  15      


Glu Tyr Leu Arg Tyr Gln Ser Asp Asn Ile Gln Gln Tyr Gly Leu Leu 
            20                  25                  30          


Gln Phe Ser Ala Asp Lys Ile Leu Asp Pro Leu Ala Gln Phe Glu Val 
        35                  40                  45              


Glu Pro Ser Lys Thr Tyr Asp Gly Leu Val His Ile Lys Ser Arg Tyr 
    50                  55                  60                  


Thr Asn Lys Tyr Leu Val Arg Trp Ser Pro Asn His Tyr Trp Ile Thr 
65                  70                  75                  80  


Ala Ser Ala Asn Glu Pro Asp Glu Asn Lys Ser Asn Trp Ala Cys Thr 
                85                  90                  95      


Leu Phe Lys Pro Leu Tyr Val Glu Glu Gly Asn Met Lys Lys Val Arg 
            100                 105                 110         


Leu Leu His Val Gln Leu Gly His Tyr Thr Glu Asn Tyr Thr Val Gly 
        115                 120                 125             


Gly Ser Phe Val Ser Tyr Leu Phe Ala Glu Ser Ser Gln Ile Asp Thr 
    130                 135                 140                 


Gly Ser Lys Asp Val Phe His Val Ile Asp Trp Lys Ser Ile Phe Gln 
145                 150                 155                 160 


Phe Pro Lys Thr Tyr Val Thr Phe Lys Gly Asn Asn Gly Lys Tyr Leu 
                165                 170                 175     


Gly Val Ile Thr Ile Asn Gln Leu Pro Cys Leu Gln Phe Gly Tyr Asp 
            180                 185                 190         


Asn Leu Asn Asp Pro Lys Val Ala His Gln Met Phe Val Thr Ser Asn 
        195                 200                 205             


Gly Thr Ile Cys Ile Lys Ser Asn Tyr Met Asn Lys Phe Trp Arg Leu 
    210                 215                 220                 


Ser Thr Asp Asn Trp Ile Leu Val Asp Gly Asn Asp Pro Arg Glu Thr 
225                 230                 235                 240 


Asn Glu Ala Ala Ala Leu Phe Arg Ser Asp Val His Asp Phe Asn Val 
                245                 250                 255     


Ile Ser Leu Leu Asn Met Gln Lys Thr Trp Phe Ile Lys Arg Phe Thr 
            260                 265                 270         


Ser Gly Lys Pro Glu Phe Ile Asn Cys Met Asn Ala Ala Thr Gln Ile 
        275                 280                 285             


Val Asp Glu Thr Ala Ile Leu Glu Ile Ile Glu Leu Gly Ser Asn Asn 
    290                 295                 300                 


<210>  7
<211>  533
<212>  PRT
<213>  Arabidopsis thaliana


<220>
<221>  PEPTIDE
<222>  (1)..(533)
<223>  D-AtCGS Mutated form of Arabidopsis cystathionine c-synthase

<400>  7

Met Ala Val Ser Ser Phe Gln Cys Pro Thr Ile Phe Ser Ser Ser Ser 
1               5                   10                  15      


Ile Ser Gly Phe Gln Cys Arg Ser Asp Pro Asp Leu Val Gly Ser Pro 
            20                  25                  30          


Val Gly Gly Ser Ser Arg Arg Arg Val His Ala Ser Ala Gly Ile Ser 
        35                  40                  45              


Ser Ser Phe Thr Gly Asp Ala Gly Leu Ser Ser Arg Ile Leu Arg Phe 
    50                  55                  60                  


Pro Pro Asn Phe Val Arg Gln Leu Ser Ile Lys Ala Arg Arg Asn Cys 
65                  70                  75                  80  


Ser Asn Ile Gly Val Ala Gln Ile Val Ala Ala Lys Trp Ser Asn Asn 
                85                  90                  95      


Pro Ser Ser Ala Ala Pro Val Ala Ala Ala Pro Pro Val Val Leu Lys 
            100                 105                 110         


Ser Val Asp Glu Glu Val Val Val Ala Glu Glu Gly Ile Arg Glu Lys 
        115                 120                 125             


Ile Gly Ser Val Gln Leu Thr Asp Ser Lys His Ser Phe Leu Ser Ser 
    130                 135                 140                 


Asp Gly Ser Leu Thr Val His Ala Gly Glu Arg Leu Gly Arg Gly Ile 
145                 150                 155                 160 


Val Thr Asp Ala Ile Thr Thr Pro Val Val Asn Thr Ser Ala Tyr Phe 
                165                 170                 175     


Phe Lys Lys Thr Ala Glu Leu Ile Asp Phe Lys Glu Lys Arg Ser Val 
            180                 185                 190         


Ser Phe Glu Tyr Gly Arg Tyr Gly Asn Pro Thr Thr Val Val Leu Glu 
        195                 200                 205             


Asp Lys Ile Ser Ala Leu Glu Gly Ala Glu Ser Thr Leu Val Met Ala 
    210                 215                 220                 


Ser Gly Met Cys Ala Ser Thr Val Met Leu Leu Ala Leu Val Pro Ala 
225                 230                 235                 240 


Gly Gly His Ile Val Thr Thr Thr Asp Cys Tyr Arg Lys Thr Arg Ile 
                245                 250                 255     


Phe Met Glu Asn Phe Leu Pro Lys Leu Gly Ile Thr Val Thr Val Ile 
            260                 265                 270         


Asp Pro Ala Asp Ile Ala Gly Leu Glu Ala Ala Val Asn Glu Phe Lys 
        275                 280                 285             


Val Ser Leu Phe Phe Thr Glu Ser Pro Thr Asn Pro Phe Leu Arg Cys 
    290                 295                 300                 


Val Asp Ile Glu Leu Val Ser Lys Ile Cys His Lys Arg Gly Thr Leu 
305                 310                 315                 320 


Val Cys Ile Asp Gly Thr Phe Ala Thr Pro Leu Asn Gln Lys Ala Leu 
                325                 330                 335     


Ala Leu Gly Ala Asp Leu Val Val His Ser Ala Thr Lys Tyr Ile Gly 
            340                 345                 350         


Gly His Asn Asp Val Leu Ala Gly Cys Ile Cys Gly Ser Leu Lys Leu 
        355                 360                 365             


Val Ser Glu Ile Arg Asn Leu His His Val Leu Gly Gly Thr Leu Asn 
    370                 375                 380                 


Pro Asn Ala Ala Tyr Leu Ile Ile Arg Gly Met Lys Thr Leu His Leu 
385                 390                 395                 400 


Arg Val Gln Gln Gln Asn Ser Thr Ala Phe Arg Met Ala Glu Ile Leu 
                405                 410                 415     


Glu Ala His Pro Lys Val Ser His Val Tyr Tyr Pro Gly Leu Pro Ser 
            420                 425                 430         


His Pro Glu His Glu Leu Ala Lys Arg Gln Met Thr Gly Phe Gly Gly 
        435                 440                 445             


Val Val Ser Phe Glu Ile Asp Gly Asp Ile Glu Thr Thr Ile Lys Phe 
    450                 455                 460                 


Val Asp Ser Leu Lys Ile Pro Tyr Ile Ala Pro Ser Phe Gly Gly Cys 
465                 470                 475                 480 


Glu Ser Ile Val Asp Gln Pro Ala Ile Met Ser Tyr Trp Asp Leu Pro 
                485                 490                 495     


Gln Glu Glu Arg Leu Lys Tyr Gly Ile Lys Asp Asn Leu Val Arg Phe 
            500                 505                 510         


Ser Phe Gly Val Glu Asp Phe Glu Asp Val Lys Ala Asp Ile Leu Gln 
        515                 520                 525             


Ala Leu Glu Ala Ile 
    530             


<210>  8
<211>  914
<212>  DNA
<213>  Anabeana


<220>
<221>  misc_feature
<222>  (1)..(914)
<223>  HetR gene from Anabaena sp. modified according to Chlamydomonas 
       reinhardtii codon usage

<400>  8
atggccggcc tgcccgtgat catgtgcctg aagagcaaca acaaccagga gtacctgcgc       60

taccagagcg acaacatcca gcagtacggc ctgctgcagt tcagcgccga caagatcctg      120

gaccccctgg cccagttcga ggtggagccc agcaagacct acgacggcct ggtgcacatc      180

aagagccgct acaccaacaa gtacctggtg cgctggagcc ccaaccacta ctggatcacc      240

gccagcgcca acgagcccga cgagaacaag agcaactggg cctgcaccct gttcaagccc      300

ctgtacgtgg aggagggcaa catgaagaag gtgcgcctgc tgcacgtgca gctgggccac      360

tacaccgaga actacaccgt gggcggcagc ttcgtgagct acctgttcgc cgagagcagc      420

cagatcgaca ccggcagcaa ggacgtgttc cacgtgatcg actggaagag catcttccag      480

ttccccaaga cctacgtgac cttcaagggc aacaacggca agacctgggc gtgatcacca      540

tcaaccagct gccctgcctg cagttcggct acgacaacct gaacgacccc aaggtggccc      600

accagatgtt cgtgaccagc aacggcacca tctgcatcaa gagcaactac atgaacaagt      660

tctggcgcct gagcaccgac aactggatcc tggtggacgg caacgacccc cgcgagacca      720

acgaggccgc cgccctgttc cgcagcgacg tgcacgactt caacgtgatc agcctgctga      780

acatgcagaa gacctggttc atcaagcgct tcaccagcgg caagcccgag ttcatcaact      840

gcatgaacgc cgccacccag atcgtggacg agaccgccat cctggagatc atcgagctgg      900

gcagcaacaa ctaa                                                        914


<210>  9
<211>  178
<212>  PRT
<213>  Zea mays


<220>
<221>  PEPTIDE
<222>  (1)..(178)
<223>  Zea mays delta zein structural 15 protein

<400>  9

Met Lys Met Val Ile Val Leu Val Val Cys Leu Ala Leu Ser Ala Ala 
1               5                   10                  15      


Ser Ala Ser Ala Met Gln Met Pro Cys Pro Cys Ala Gly Leu Gln Gly 
            20                  25                  30          


Leu Tyr Gly Ala Gly Ala Gly Leu Thr Thr Met Met Gly Ala Gly Gly 
        35                  40                  45              


Leu Tyr Pro Tyr Ala Glu Tyr Leu Arg Gln Pro Gln Cys Ser Pro Leu 
    50                  55                  60                  


Ala Ala Ala Pro Tyr Tyr Ala Gly Cys Gly Gln Pro Ser Ala Met Phe 
65                  70                  75                  80  


Gln Pro Leu Arg Gln Gln Cys Cys Gln Gln Gln Met Arg Met Met Asp 
                85                  90                  95      


Val Gln Ser Val Ala Gln Gln Leu Gln Met Met Met Gln Leu Glu Arg 
            100                 105                 110         


Ala Ala Ala Ala Ser Ser Ser Leu Tyr Glu Pro Ala Leu Met Gln Gln 
        115                 120                 125             


Gln Gln Gln Leu Leu Ala Ala Gln Gly Leu Asn Pro Met Ala Met Met 
    130                 135                 140                 


Met Ala Gln Asn Met Pro Ala Met Gly Gly Leu Tyr Gln Tyr Gln Leu 
145                 150                 155                 160 


Pro Ser Tyr Arg Thr Asn Pro Cys Gly Val Ser Ala Ala Ile Pro Pro 
                165                 170                 175     


Tyr Tyr 
        


<210>  10
<211>  2603
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(2603)
<223>  D-AtCGS under the control of Chlamydomonas chloroplast atpA pro 
       and rbcL te

<400>  10
ggatccgact ttattagagg cagtgtttat atacctaaac gtcaaaagtc atttttataa       60

ctggtctcaa aatacctata aacccattgt tcttctcttt tagctctaag aacaatcaat      120

ttataaatat atttattatt atgctataat ataaatacta tataaataca tttacctttt      180

tataaataca tttacctttt ttttaatttg catgatttta atgcttatgc tatctttttt      240

atttagtcca taaaaccttt aaaggacctt ttcttatggg atatttatat tttcctaaca      300

aagcaatcgg cgtcataaac tttagttgct tacgacgcct gtggacgtcc cccccttccc      360

cttacgggca agtaaactta gggattttaa tgcaataaat aaatttgtcc tcttcgggca      420

aatgaatttt agtatttaaa tatgacaagg gtgaaccatt acttttgtta acaagtgatc      480

ttaccactca ctatttttgt tgaattttaa acttatttaa aattctcgag aaagatttta      540

aaaataaact tttttaatct tttatttatt ttttcttttt tatggcaatg cgtactccag      600

aagaacttag taatcttatt aaagatttaa ttgaacaata cactccagaa gtgaaacata      660

tgcgtcagct gagcattaaa gcccgtagaa actgtagcaa catcggtgtt gcacagatcg      720

tggcggctaa gtggtccaac aacccatcct ccgccgcccc tgtggctgcc gcgcctcccg      780

tcgtgctgaa aagcgtcgat gaggaggttg tggtggccga ggaggggatc agggagaaga      840

taggtagtgt acagctgacg gattccaaac attctttctt gagctccgat gggagcctca      900

ctgttcatgc cggtgaaaga ttaggccgtg gtatagtgac ggatgctatc actactcctg      960

tagtcaacac atctgcctac ttcttcaaga aaactgctga gcttattgac ttcaaggaga     1020

aaaggagtgt gagtttcgag tatggtcgtt atggtaaccc tacgactgtg gtacttgaag     1080

ataagataag tgcacttgaa ggggctgaat caactttggt tatggcatct gggatgtgtg     1140

caagcactgt tatgcttttg gcattggttc ctgctggtgg acacattgtc actactactg     1200

attgctacag gaagactagg atcttcatgg agaattttct tcccaagttg gggatcactg     1260

tcactgtgat tgatcctgct gatatcgcag ggcttgaagc tgcagtgaat gagttcaagg     1320

tatctctgtt cttcactgag tccccgacaa acccattcct tagatgtgtc gacattgagc     1380

tagtttcaaa aatatgccac aagaggggaa ctctggtttg cattgatggc acctttgcaa     1440

cacctctgaa tcagaaagcc cttgctcttg gtgctgatct tgtcgtgcac tctgctacaa     1500

agtacattgg aggacacaat gatgttcttg ctggatgcat ctgtggttca ctgaagttgg     1560

tttcagaaat tcgcaatctg catcatgtgt tgggaggaac acttaaccca aacgctgcgt     1620

acctaatcat ccgaggcatg aagacattgc atcttcgtgt acagcaacag aattcgaccg     1680

cttttagaat ggccgaaatt ttagaggcac atcctaaggt gagtcatgtg tactatccag     1740

gccttccaag tcatcccgaa catgaactcg ccaagcgaca aatgactggt tttggaggtg     1800

tggtcagttt cgagattgat ggagacattg aaacgacaat caagtttgtg gattctctaa     1860

agattcctta cattgcacca tccttcggtg gctgcgaaag cattgttgac caacctgcta     1920

tcatgtccta ctgggatctg ccgcaagagg agagactaaa gtatggaatc aaagataact     1980

tggttcgttt cagctttgga gttgaagact ttgaagatgt caaagctgac attcttcaag     2040

ctctcgaagc catctaccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc     2100

cggactatgc aggatcctat ccatatgacg ttccagatta cgctgctcag tagtctagat     2160

ttttattttt catgatgttt atgtgaatag cataaacatc gtttttattt tttatggtgt     2220

ttaggttaaa tacctaaaca tcattttaca tttttaaaat taagttctaa agttatcttt     2280

tgtttaaatt tgcctgtgct ttataaatta cgatgtgcca gaaaaataaa atcttagctt     2340

tttattatag aatttatctt tatgtattat attttataag taataaaaga aatagtaaca     2400

tactaaagcg gatgtaactc aatcggtaga gtgcgatcct tccaagttcg aggttgtggg     2460

ttcgagtccc atcatccgct aaaccaatct ataaaagttg ttgaatatgc tgaaatgttt     2520

tcaaagaaaa agcctagttt ttcttttaca acaagcaaag aacaattggc attctttgat     2580

tgtaagaaaa tgcgcttgga tcc                                             2603


<210>  11
<211>  1238
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(1238)
<223>  HetR gene from Anabaena sp. linked to the alfa-helical coiled 
       coil high lysine protein, modified according to Chlamydomonas 
       reinhardtii codon usage

<400>  11
aagcttttcg aaatgtccaa cgatattgat ctgattaagc ggctgggtcc gtcggccatg       60

gaccagatca tgctgtacct ggccttcagc gcgatgcgga cgagcggcca ccgccacggc      120

gccttcctcg acgctgccgc gacggctgcg aagtgcgcga tctacatgac ctacctcgag      180

cagggccaga acctccgcat gaccggccac ctccaccacc tcgagcccaa gcgcgtcaag      240

atcattgtcg aggaggtccg gcaagccctg atggagggca agctgctcaa gaccctgggg      300

agccaggagc cccgctacct gatccagttc ccctacgtgt ggatggagca gtacccgtgg      360

attcccgggc ggtcccgcat ccccggcacc agcctgacgt cggaggagaa gcgccagatc      420

gagcacaagc tgccgagcaa cctgccggac gcgcagctgg tgacctcgtt tgagtttctg      480

gagctgatcg agtttctgca caagcggagc caggaggacc tgcctccgga gcatcgcatg      540

gagctgtcgg aggcgctggc ggagcacatc aagcgccgcc tgctgtactc cggcacggtg      600

acccgcatcg actccccctg gggtatgccc ttctatgcgc tgactcgccc cttctacgct      660

cccgccgacg atcaggagcg gacctacatc atggtggagg acactgcccg ctacttccgc      720

atgatgaagg actgggccga gaagcgcccc aacgcgatgc gcgctctgga ggagctcgac      780

gtgcctcccg agcgctggga cgaggccatg caagagctgg acgagatcat ccgcacgtgg      840

gccgacaagt accaccaggt gggcggcatc ccgatgattc tgcagatggt gttcggccgc      900

aaggaggacg tgccgtcgac gccgcctacc ccgtccccga gcacccctcc caccccctcc      960

ccctcgatgg aggagaagct gaaggccatg gaggagaagc tgaaggctat ggaggagaag     1020

ctgaaggcca tggaggagaa gctgaaggcg atggaggaga agctgaaggc catggaggag     1080

aagctgaagg ccatggagga gaagatgaag gcgctggagt atccctacga cgtgcccgac     1140

tacgcgggct acccctacga cgtgcccgac tacgccggca gctacccgta cgacgtgccg     1200

gactacgccg ctcagtaact cgagtgagat ccggatcc                             1238


<210>  12
<211>  135
<212>  PRT
<213>  Chlamydomonas


<220>
<221>  PEPTIDE
<222>  (1)..(135)
<223>  Chlamydomonas rbS transit peptide

<400>  12

Ala Thr Gly Gly Cys Cys Gly Cys Cys Gly Thr Cys Ala Thr Thr Gly 
1               5                   10                  15      


Cys Cys Ala Ala Gly Thr Cys Cys Thr Cys Cys Gly Thr Cys Thr Cys 
            20                  25                  30          


Cys Gly Cys Gly Gly Cys Cys Gly Thr Gly Gly Cys Cys Cys Gly Cys 
        35                  40                  45              


Cys Cys Gly Gly Cys Cys Cys Gly Cys Thr Cys Cys Ala Gly Cys Gly 
    50                  55                  60                  


Thr Gly Cys Gly Cys Cys Cys Cys Ala Thr Gly Gly Cys Cys Gly Cys 
65                  70                  75                  80  


Gly Cys Thr Gly Ala Ala Gly Cys Cys Cys Gly Cys Cys Gly Thr Cys 
                85                  90                  95      


Ala Ala Gly Gly Cys Cys Gly Cys Cys Cys Cys Cys Gly Thr Gly Gly 
            100                 105                 110         


Cys Thr Gly Cys Cys Cys Cys Gly Gly Cys Thr Cys Ala Gly Gly Cys 
        115                 120                 125             


Cys Ala Ala Cys Cys Ala Gly 
    130                 135 


<210>  13
<211>  99
<212>  PRT
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  PEPTIDE
<222>  (1)..(99)
<223>  3xHA epitope tag according ot Chlamydomonas codon usage

<400>  13

Thr Ala Cys Cys Cys Gly Thr Ala Cys Gly Ala Thr Gly Thr Cys Cys 
1               5                   10                  15      


Cys Gly Gly Ala Cys Thr Ala Cys Gly Cys Cys Gly Gly Cys Thr Ala 
            20                  25                  30          


Thr Cys Cys Cys Thr Ala Cys Gly Ala Thr Gly Thr Gly Cys Cys Thr 
        35                  40                  45              


Gly Ala Cys Thr Ala Cys Gly Cys Gly Gly Gly Cys Thr Cys Cys Thr 
    50                  55                  60                  


Ala Cys Cys Cys Cys Thr Ala Cys Gly Ala Cys Gly Thr Gly Cys Cys 
65                  70                  75                  80  


Cys Gly Ala Cys Thr Ala Cys Gly Cys Thr Gly Cys Cys Cys Ala Gly 
                85                  90                  95      


Thr Ala Gly 
            


<210>  14
<211>  739
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (1)..(739)

<220>
<221>  misc_feature
<222>  (1)..(739)
<223>  gene encoding Zea mays delta zein structural 15 protein

<400>  14
tccagatcag caaagcggca gtgcgtagag aggatcgtcg aacagaacag catgaagatg       60

gtcatcgttc tcgtcgtgtg cctggctctg tcagctgcca gcgcctctgc aatgcagatg      120

ccctgcccct gcgcggggct gcagggcttg tacggcgctg gcgccggcct gacgacgatg      180

atgggcgccg gcgggctgta cccctacgcg gagtacctga ggcagccgca gtgcagcccg      240

ctggcggcgg cgccctacta cgccgggtgt gggcagccga gcgccatgtt ccagccgctc      300

cggcaacagt gctgccagca gcagatgagg atgatggacg tgcagtccgt cgcgcagcag      360

ctgcagatga tgatgcagct tgagcgtgcc gctgccgcca gcagcagcct gtacgagcca      420

gctctgatgc agcagcagca gcagctgctg gcagcccagg gtctcaaccc catggccatg      480

atgatggcgc agaacatgcc ggccatgggt ggactctacc agtaccagct gcccagctac      540

cgcaccaacc cctgtggcgt ctccgctgcc attccgccct actactgatt catgatattt      600

gggaaatctc ctctatccat ctctctctat ctatatatgt aataatgcag taagacgaca      660

cacattatca tgtgtggtat gaccaataat atatgcatgg tcataataaa gttttggttt      720

taaaaaaaaa aaaaaaaaa                                                   739


<210>  15
<211>  1146
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(1146)
<223>  15kD Zein fused to 10kD Zzein and 3xHA, Chlamydomonas codon 
       usage)

<400>  15
atgaagatgg tgatcgtgct cgtcgtgtgc ctcgccctgt ccgcggctag cgcgagcgcc       60

atgcagatgc cgtgcccctg cgctggcctg cagggcctgt acggtgcggg tgccggcctg      120

acgacgatga tgggggctgg cgggctgtac ccgtacgccg agtacctccg gcagccccag      180

tgctcgccgc tggccgctgc cccctactat gcgggctgcg gccagcccag cgccatgttc      240

caacccctgc gccagcagtg ctgccagcag cagatgcgca tgatggacgt ccagagcgtg      300

gcgcagcagc tgcaaatgat gatgcagctg gagcgcgctg ctgccgcctc cagctccctg      360

tacgagcccg ctctgatgca gcagcagcaa cagctgctgg ctgcccaggg cctgaacccc      420

atggcgatga tgatggccca gaacatgccc gcgatgggtg gcctgtacca gtaccagctg      480

ccctcgtacc gcaccaaccc gtgcggcgtg tcggctgcga tcccgcccta ctacgtgccc      540

tcgacgcccc ctaccccctc cccctcgacc cctccgaccc cttccccatc gatggcggcc      600

aagatgctgg cgctgtttgc gctgctggcc ctgtgcgcct cggccaccag cgccactcac      660

attcccggcc acctgcctcc ggtgatgccc ctgggcacga tgaacccgtg catgcaatac      720

tgcatgatgc aacagggcct ggccagcctc atggcgtgcc cgtccctgat gctccaacag      780

ctcctggccc tgcccctgca gaccatgccg gtgatgatgc cccagatgat gaccccgaac      840

atgatgtcgc cgctgatgat gcctagcatg atgagcccca tggtcctgcc ctccatgatg      900

agccagatga tgatgccgca gtgccactgc gacgcggtga gccagatcat gctgcagcag      960

cagctcccct tcatgttcaa cccgatggcc atgaccatcc cgcctatgtt tctgcagcag     1020

cccttcgtgg gcgccgcctt tctcgagtac ccgtacgacg tccctgacta cgcgggctac     1080

ccctacgatg tgcccgacta cgcgggctcc tatccctacg acgtgccgga ctacgctgcg     1140

cagtga                                                                1146


<210>  16
<211>  534
<212>  DNA
<213>  Zea mays


<220>
<221>  CDS
<222>  (1)..(534)
<223>  15kD Zein according to Chlamydomonas chloroplast codon usage

<400>  16
atg aaa atg gta att gtt ctt gta gtt tgt tta gca tta agt gct gca         48
Met Lys Met Val Ile Val Leu Val Val Cys Leu Ala Leu Ser Ala Ala           
1               5                   10                  15                

tca gct agt gca atg caa atg cct tgt cca tgt gct ggt tta caa ggt         96
Ser Ala Ser Ala Met Gln Met Pro Cys Pro Cys Ala Gly Leu Gln Gly           
            20                  25                  30                    

tta tat ggt gct ggt gct gga tta act aca atg atg gga gct ggt ggt        144
Leu Tyr Gly Ala Gly Ala Gly Leu Thr Thr Met Met Gly Ala Gly Gly           
        35                  40                  45                        

tta tat cct tat gct gaa tat tta cgt caa cca caa tgt tct cca tta        192
Leu Tyr Pro Tyr Ala Glu Tyr Leu Arg Gln Pro Gln Cys Ser Pro Leu           
    50                  55                  60                            

gct gca gct cca tat tac gca ggt tgt ggt caa cca agt gct atg ttt        240
Ala Ala Ala Pro Tyr Tyr Ala Gly Cys Gly Gln Pro Ser Ala Met Phe           
65                  70                  75                  80            

caa cca tta cgt caa caa tgt tgt caa caa caa atg cgt atg atg gat        288
Gln Pro Leu Arg Gln Gln Cys Cys Gln Gln Gln Met Arg Met Met Asp           
                85                  90                  95                

gtt caa tct gta gct caa caa tta caa atg atg atg caa tta gaa aga        336
Val Gln Ser Val Ala Gln Gln Leu Gln Met Met Met Gln Leu Glu Arg           
            100                 105                 110                   

gca gct gca gct tca agt tca tta tat gaa cct gca tta atg caa caa        384
Ala Ala Ala Ala Ser Ser Ser Leu Tyr Glu Pro Ala Leu Met Gln Gln           
        115                 120                 125                       

caa caa caa tta ctt gct gca caa ggt tta aat cct atg gct atg atg        432
Gln Gln Gln Leu Leu Ala Ala Gln Gly Leu Asn Pro Met Ala Met Met           
    130                 135                 140                           

atg gct caa aat atg cct gct atg ggt ggt tta tat caa tac caa tta        480
Met Ala Gln Asn Met Pro Ala Met Gly Gly Leu Tyr Gln Tyr Gln Leu           
145                 150                 155                 160           

cca agt tat cgt aca aac cca tgt ggt gta tct gct gct att cca cca        528
Pro Ser Tyr Arg Thr Asn Pro Cys Gly Val Ser Ala Ala Ile Pro Pro           
                165                 170                 175               

tat tac                                                                534
Tyr Tyr                                                                   
                                                                          


<210>  17
<211>  178
<212>  PRT
<213>  Zea mays

<400>  17

Met Lys Met Val Ile Val Leu Val Val Cys Leu Ala Leu Ser Ala Ala 
1               5                   10                  15      


Ser Ala Ser Ala Met Gln Met Pro Cys Pro Cys Ala Gly Leu Gln Gly 
            20                  25                  30          


Leu Tyr Gly Ala Gly Ala Gly Leu Thr Thr Met Met Gly Ala Gly Gly 
        35                  40                  45              


Leu Tyr Pro Tyr Ala Glu Tyr Leu Arg Gln Pro Gln Cys Ser Pro Leu 
    50                  55                  60                  


Ala Ala Ala Pro Tyr Tyr Ala Gly Cys Gly Gln Pro Ser Ala Met Phe 
65                  70                  75                  80  


Gln Pro Leu Arg Gln Gln Cys Cys Gln Gln Gln Met Arg Met Met Asp 
                85                  90                  95      


Val Gln Ser Val Ala Gln Gln Leu Gln Met Met Met Gln Leu Glu Arg 
            100                 105                 110         


Ala Ala Ala Ala Ser Ser Ser Leu Tyr Glu Pro Ala Leu Met Gln Gln 
        115                 120                 125             


Gln Gln Gln Leu Leu Ala Ala Gln Gly Leu Asn Pro Met Ala Met Met 
    130                 135                 140                 


Met Ala Gln Asn Met Pro Ala Met Gly Gly Leu Tyr Gln Tyr Gln Leu 
145                 150                 155                 160 


Pro Ser Tyr Arg Thr Asn Pro Cys Gly Val Ser Ala Ala Ile Pro Pro 
                165                 170                 175     


Tyr Tyr 
        


<210>  18
<211>  813
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(813)
<223>  15kD Zzein fused to 3xHA, according to Phaeodactylum tricornutum 
       codon usage

<400>  18
atgagatcct tttgcatcgc agcccttttg gctgtggcat ctgccttcac cacacagcca       60

acttccttca ctgtgaagac tgcgaatgtg ggcgaacggg cgagtggggt tttccctgag      120

cagtcttctg ctcatcgcac gcgtaaagca acgattgtca tgaagatggt catcgtcctc      180

gtcgtctgcc tcgccttgtc cgccgcctcc gcctccgcca tgcagatgcc ctgtccctgc      240

gccggactcc agggactcta cggtgccggt gccggactca ccaccatgat gggagccggt      300

ggactctacc cctacgccga atacctccgt cagccccagt gctccccttt ggccgccgcc      360

ccctactacg ccggatgtgg acagccgtcc gccatgttcc agcccttgcg tcagcagtgc      420

tgccagcagc agatgcgtat gatggacgtc cagtccgtcg cccagcagct ccagatgatg      480

atgcagctcg aacgtgccgc cgccgcctcc tcctccttgt acgaacccgc cctcatgcag      540

cagcagcaac agttgctcgc cgcccaggga ctcaacccca tggccatgat gatggcccag      600

aacatgcccg ccatgggagg actctaccag taccagctcc cctcctaccg taccaacccc      660

tgtggtgtct ccgccgccat tcccccgtac tacagatctg gtggaggtgg cggatacccg      720

tacgacgtcc ctgattacgc cggataccct tacgatgtcc cggactacgc cggttcctac      780

ccctacgacg tcccggacta cgccgcccag taa                                   813


<210>  19
<211>  34
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(34)
<223>  Fw primer

<400>  19
cgaattcttc gaaatgaaga tggtgatcgt gctc                                   34


<210>  20
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(36)
<223>  reverse primer

<400>  20
cggatcctca ctcgaggtag tagggcggga tcgcag                                 36


<210>  21
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  forward primer

<400>  21
cccgcatctt catggagaac                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  reverse primer

<400>  22
gtgaccgccg atgtacttag                                                   20


<210>  23
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  forward primer

<400>  23
atgagtaacg acatcgatct g                                                 21


<210>  24
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  chemically synthetized


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  reverse primer

<400>  24
ttaatcttct tttctaccaa acac                                              24


